A simplified assay method for galactosyl ceramide β-galactosidase
نویسندگان
چکیده
منابع مشابه
The inhibitor-sensitive sites of galactosyl ceramide galactosidase.
A preparation of galactosyl ceramide galactosidase from rat brain has been tested with potential inhibitors which resemble the enzyme’s substrate. The amide made from 2-hydroxydodecanoic acid and nL-erythro-3-phenyl-2-amino-1,3-propanediol proved to be a fine inhibitor, acting noncompetitively. Removal of any of the three hydroxyl groups reduced the effectiveness, as did inversion of the C-3 ca...
متن کاملSynthesis of galactosyl ceramide and glucosyl ceramide by rat brain: assay procedures and changes with age.
Galactosyl ceramide is made in mouse brain from ceramide and uridine diphospho galactose (UDPGal)16,1L The enzyme system, evaluated in vitro, shows a marked preference for 2-hydroxy fatty acids (HFA) ceramide when compared with nonhydroxy fatty acids (NFA) ceramide, although brain contains HFA and NFA cerebroside in similar amounts. We do not yet know whether this difference is due to enzyme sp...
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Bionic acids are bioactive compounds demonstrating numerous interesting properties. They are widely produced by chemical or enzymatic oxidation of disaccharides. This paper focuses on the galactosyl derivative of gluconic acid as a result of a new method of bionic acid synthesis which utilises the transglycosylation properties of β-galactosidase and introduces lactose as a substrate. Products o...
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mouse genotyping by the three-primer multiple PCR. However, both of the smaller PCR products of 1068 (specific for the wild-type allele of mGluR4) and 1170 bp (specific for the mGluR4 transgene) were visible. Thus, all three allele combinations were clearly distinguishable on agarose gels stained with ethidium bromide. DNA from wildtype mice give a single smaller band (1068 bp; see Figure 2B, l...
متن کاملA fluorescent assay for ceramide synthase activity.
The sphingolipids are a diverse family of lipids with important roles in membrane compartmentalization, intracellular signaling, and cell-cell recognition. The central sphingolipid metabolite is ceramide, formed by the transfer of a variable length fatty acid from coenzyme A to a sphingoid base, generally sphingosine or dihydrosphingosine (sphinganine) in mammals. This reaction is catalyzed by ...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1971
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)39536-5